FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344106

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344106
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2503153
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12250.037.375781
TCTGTCG12400.036.573368
TCGCTCT13700.033.628344
CGCTCTG14650.031.9390325
GCGTAAC1203.6445123E-430.7697091
CTCGCTC14400.029.9946253
GTCTTGC22750.029.214321
CTGTCGC16900.028.9646479
TCTCGCT16350.028.1981542
GTATAAC4100.027.0173071
GTGTTAT7750.026.6803171
GTATTAG7750.024.774581
GTACGGG1801.3424987E-424.6157681
TTGCTCT33850.023.6053454
TACACTG14700.023.5055265
GTGTAGT14350.023.157691
TTACACT12550.022.943644
GTATTAC5350.022.0851751
GTCTCAC33300.021.9546031
GTGTTAC10200.021.7197951
Produced by FastQC (version 0.11.5)