FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344110

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344110
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3221850
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14050.038.9443248
GTCTCGC14800.036.5791441
CTGTCGC18950.031.533769
GTCTTGC31450.031.2142011
TCGCTCT16150.029.8691374
TCTCGCT18650.026.2577972
GCGTTAA1658.6792366E-526.2483061
CTCGCTC17950.026.0721023
GTACTAT7300.025.7089561
GTGTTAC15700.024.8272191
GTCTTAC14150.024.4860531
CGCTCTG18650.023.9349885
GTACTAG7250.023.895011
CCGTTCG1851.9206229E-423.3499729
GTATTAC8700.023.231261
GTATAGA11000.022.9672681
GGTATAC6350.022.7347531
GTGTAAA20300.022.045991
TTGCTCT45650.021.9227184
TATACTG10600.021.7352435
Produced by FastQC (version 0.11.5)