FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271335
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.052.1489878
GTCTCGC9550.043.1848261
CTGTCGC12000.040.1981749
TCGCTCT11800.038.4372374
TCTCGCT11350.034.2939642
GTCTTGC23700.032.9712261
CTCGCTC12050.031.7036043
GTGTTAC8350.031.1943381
GTATAGT6400.029.3936211
GTATTAT8150.028.4087491
TTGCTCT30800.026.6469694
CGCTCTG13400.026.3260045
GTATTGG8250.024.5563471
GTCTCAC27700.024.291981
GTGTAAT9900.024.1178421
GTGTAAA12900.024.1178421
CGTTTCG1500.00155673523.998919
CTTGCTC29650.023.8243583
GGGTTTC39800.023.8148541
GTATAAT7650.023.6449451
Produced by FastQC (version 0.11.5)