FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271335
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10650.043.9410678
GTCTCGC10900.035.077381
CTGTCGC14250.033.8506369
GTATTAG7750.033.5103231
ACCGTGT6950.033.149128
GTCTTGC22100.031.6633781
TCGCTCT11450.031.4391944
GGGTTTC38650.029.1174181
GTTTCAC36350.028.5210113
TTCACCG7250.026.812215
CCGTGTT8200.026.339919
TCTCGCT13150.026.283292
GGTTTCA37800.026.0971472
GTGTAAC6850.024.2222821
TCTCGAT9900.023.9985857
CTCGATC9750.023.6293748
GTCTTAC9500.023.5405121
TCGATCT9850.023.3894829
TCCGATC1851.9203806E-423.3499723
GTATTAT6800.023.33951
Produced by FastQC (version 0.11.5)