FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344117

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344117
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058359
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9600.039.7471248
GTCTCGC8500.037.4544031
TCGCTCT9900.036.3610044
CGTTTCG1301.73678E-533.2283639
CTGTCGC11750.033.0869649
GTCTTGC22000.030.5864721
CGCTCTG11900.029.0399135
GTATAAC4400.027.9553781
GTATAAT6750.027.8701131
TATTGCG1856.1451683E-627.2412729
TCTCGCT11800.026.8775062
CTCGCTC11550.026.8351963
TATACTG6050.026.1799245
TTATACT7000.024.683934
CTATACT5950.024.1999284
GTATTAT6950.023.944851
GTGTAAT8850.023.709581
TACTCCG1851.9204694E-423.349665
GTACTAT5450.022.5694791
AGTACCG1600.00226616722.4983715
Produced by FastQC (version 0.11.5)