FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344118

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344118
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2058359
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.039.337478
GCGCAAC1402.8401697E-530.9218561
CTGTCGC11900.030.8559
GTCTCGC9150.030.7528821
GTACCGA1607.059712E-527.0566221
GTCTTGC20950.026.1741071
GTATTAC5000.025.9743561
CGCTCTG10750.025.450015
TCGCTCT10500.025.3702834
GTATAGG4600.025.0959971
GTATAGC4900.023.5595071
GTATTCG1902.2671414E-422.7845251
GTCTCAC25650.022.7845231
GTATAGA6700.022.614491
GTGTAAC7050.022.5152031
CTCGCTC11700.022.1533833
GTATTAG7200.022.0461371
GTATAGT5600.021.902981
GTGTAAG10950.021.7441351
GTATAAT6000.021.6452981
Produced by FastQC (version 0.11.5)