FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3378917
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13350.044.2221348
GTCTCGC15950.035.8434141
CTGTCGC17400.035.584139
TCGCTCT17550.033.6375664
GTCTTGC35000.031.2213631
GTATTAG13400.029.1629811
CTCGCTC17750.028.422993
GTATTAC10300.026.698651
TCTCGCT19250.026.208212
GGTATAC7100.025.4814781
GTCTCAC41000.024.8873251
CGCTCTG21250.024.7326725
GTATAAC7500.024.1224631
TTGCGCG1500.001557010523.9985969
GTGTAGG18800.023.4809111
GTATAGA9050.023.1895541
GTATAAG9800.022.8917271
GTGTTAG23350.022.3145731
CTGTTGC38750.022.2954719
GTCTAGA15600.022.2668911
Produced by FastQC (version 0.11.5)