FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344121

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344121
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677344
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG16150.042.350828
GTCTCGC14950.041.1807821
CTGTCGC18100.037.3909039
TCGCTCT17000.033.8797344
GTCTTGC35700.033.6788141
TCTCGCT18900.030.8929842
CTCGCTC19700.028.9066353
CGCTCTG19650.028.2115615
GTATTAC8800.027.9842171
TTGCTCT49600.025.5464134
GTGTTAC16350.023.4787541
GTGTAGG17900.023.4688531
GTATAGA11600.023.1025891
CTTGCTC48000.022.6763083
GTGTTAG25950.022.6081261
GTCTCAC44150.021.3269881
GTCTAAC9300.021.0279881
GTATAAT12750.021.0188261
TACACTG23450.020.8768525
GGCGTAC2104.3454906E-420.6942121
Produced by FastQC (version 0.11.5)