FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344122

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344122
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3677344
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG17100.045.8920178
GTCTCGC16750.039.6560941
TCGCTCT18800.037.148254
CTGTCGC21300.035.8288659
GTCTTGC35550.034.9321861
CTCGCTC19400.031.9169393
CGCTCTG20250.030.9327625
TCTCGCT20950.030.593622
CGTTTCG1856.1462597E-627.241639
TTGCTCT50400.025.9995214
GGGTTTC61300.025.3229871
CTTGCTC47950.025.2258133
GTATTAT13750.024.1541671
GTCTTAC15800.023.305051
GTATTAC8450.023.0698071
TACACTG23250.022.9157075
GTTTCAC58500.022.8918863
GTACCGC2252.7404341E-522.4622081
TTACACT20200.021.7421724
GTGTTAG26650.021.6735321
Produced by FastQC (version 0.11.5)