FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2603952
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11700.038.156511
TCTGTCG13400.033.8505068
TCGCTCT13700.032.0581674
CTCGCTC14450.030.394253
TCTCGCT15050.029.1825162
GTCTTGC21750.028.4709511
CGCTCTG13600.027.5292195
GTATAAG6900.026.0887761
CTGTCGC17100.025.684039
GTCTTAC8450.024.7117821
GTGCTAC7150.024.169521
TTGCTCT30850.021.4714854
GGAGTGC58150.021.0488645
ACCCGCG2404.605757E-520.9998537
GTGTAAA13550.020.7246911
GTAACGC1750.003810171520.5712833
GAGTGCA61700.020.421256
TCGAACT17000.020.3292689
GTGTTAG15000.020.1614061
TACTGCG1800.004485162419.9998577
Produced by FastQC (version 0.11.5)