FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344129

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344129
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2999389
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11700.048.6131368
CTGTCGC14800.040.8629769
TCGCTCT13400.034.930174
GTCTCGC13100.034.7163731
GTCTTGC31950.032.9873241
GTATTAT8800.031.1720961
TCTCGCT15000.029.7680552
GTGTAAC8700.029.0411551
GTATACG2156.3667903E-726.8606591
CGCTCTG16450.025.8225155
CTCGCTC15900.025.8148613
CGTTTCG1400.001041407725.7130979
GTGTTAG20350.025.1859911
GGTATAC6700.024.7809621
TTGCTCT41850.024.0893254
GTCTCAC36050.023.6288071
GTATAAC5600.023.2032891
TAATACT10150.022.7026234
GTATTAA8750.022.275161
GTGTAAG16250.022.2116971
Produced by FastQC (version 0.11.5)