FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2854798
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12700.040.818738
GTCTCGC13050.034.762131
CTGTCGC16300.033.5704159
GTCTTGC29350.031.6488511
TCGCTCT13100.030.2288844
TTCACCG7800.029.538345
GTCTTAC14550.026.7243461
GTATTAT9450.026.6693611
TCTCGCT15250.026.4392382
GTACCGT2207.9536767E-726.1844621
GTGTAAC10000.025.9226171
GTGTTAG20100.024.7189121
CTCGCTC14750.024.4066773
GTTTCAC48800.024.196623
GTATAGC6550.024.1856481
GTACTAG6650.023.8219531
CTATGCG2752.1075903E-723.563549
GTCTCAC34600.023.3087121
TTGCTCT38550.023.1594374
CGCTCTG15550.022.688015
Produced by FastQC (version 0.11.5)