FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796390
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12000.044.398418
GTCTCGC12850.043.8249631
CTGTCGC14700.037.7129369
TCGCTCT15650.036.8077474
TCTCGCT15800.036.460912
CTCGCTC16650.032.434753
CGCTCTG17850.030.6543645
GTATTAC7600.028.499531
GTATTAA8500.028.0301271
GTATTAT8750.026.4041391
GTCTTAC11650.026.0287571
GTGTTAG18650.025.9373761
GTCTTGC27300.025.9175241
GTTTCAC46200.024.3134273
GTATAGG7550.023.9068931
GGGTTTC49700.023.6788851
GGTATAC6600.022.9723511
GTATAAG9200.022.7583221
GTATTGC7400.022.440171
GTGTTAA11800.022.0267561
Produced by FastQC (version 0.11.5)