FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2796390
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.039.8767058
GTCTCGC12450.039.3264921
TCGCTCT14300.035.748064
GTCTTGC25500.034.7304651
CTGTCGC15950.031.5985749
TCTCGCT15800.030.531482
CGCTCTG15350.030.4884325
CTCGCTC16500.030.1089273
TAATACT9050.029.4363024
GTATTAT9050.027.8461481
GTATTAG8850.027.6618561
GTAATAC7350.025.469253
GTATTAA9300.024.7749441
TACACCG2452.0329535E-623.5100755
TTGCTCT38100.023.2439674
GTATACT6200.023.225684
GTTTCAC47600.023.1427333
TTCACCG10000.023.0398755
GGGTTTC49900.022.7982881
GTGTAAT11850.022.4816951
Produced by FastQC (version 0.11.5)