FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2598965
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAT1752.5338522E-937.129881
GTCTCGC10950.031.6479971
GTCTTGC20450.031.4206941
TCGCTCT11900.030.2560734
TCTGTCG10800.027.3321868
CTGTCGC12500.026.494899
CTCGCTC12650.026.1852533
CGCTCTG12800.024.7489625
AGTACCG1500.001556763723.9989955
TTGCTCT30050.023.963214
TCTCGCT13850.023.9206372
GGGTACG2451.9850377E-623.5745281
GTATTAC5350.022.94111
GAGTGCA58650.022.5872866
GTCGACC2303.318115E-521.9121238
GTATAAG8250.021.8778761
CGTCAGA4401.03682396E-1021.3309271
GGCTAGA14950.021.2486111
CTTGCTC27600.021.133213
GTGTAAC5500.021.0027621
Produced by FastQC (version 0.11.5)