FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662111
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11300.042.0515028
CTGTCGC14050.038.4327059
GTCTCGC12000.031.2851921
TCGCTCT12950.029.4710454
GTCTTGC26850.028.5021971
GCGCTAG2105.178026E-727.5034661
GTGTTAG18000.027.2742711
GTGTAAC8950.025.0066411
GTATTAA8350.024.2096391
GTGTAAA18250.024.1314661
ACCGTGT9500.023.4938458
CGCTCTG14450.023.4177975
GTACGGA1902.2577032E-422.7989271
TTACACT15800.022.7877984
TACACTG18900.022.4753385
TCTCGCT16200.021.7834972
GTGTTAT11000.021.6589811
GTGTAAT12000.021.658981
GTATAAC5001.8189894E-1221.658981
CTCGCTC15650.021.6258373
Produced by FastQC (version 0.11.5)