FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662111
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.041.5998348
GTCTCGC12850.034.7404671
TCGCTCT13950.032.5160034
CTGTCGC15400.032.2596179
GTCTTGC24800.031.0655611
CGCTCTG15350.028.1432135
GTTCGTA1555.7681944E-527.8719061
TTTACCG1555.7696707E-527.870865
GTATTAC7850.027.516851
GTATTAA9200.027.3922271
TCTCGCT15900.027.1697062
CTCGCTC15400.026.6492483
GTGTACG2901.2831151E-824.828421
GTCTCAC32350.024.4830471
TAATACT8300.024.289064
GGTAACG2452.0328644E-623.5101132
GTATAAG9250.023.3521391
GGTATAC6800.023.29491
GTATTAT9050.023.0725981
GTCTTAC10350.022.9572941
Produced by FastQC (version 0.11.5)