FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2690555
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10700.034.9892588
GTCTCGC12600.033.2341771
TCGCTCT14450.030.8983944
CTGTCGC13050.030.3436189
CGCTCTG14750.026.8463865
GTACCGT1358.4217713E-426.6656046
CTCGCTC15100.026.229973
TCTCGCT15700.026.1483172
GTCTTGC24900.025.2259431
GTATAAT5750.025.1124821
GTATAAG5400.024.0661281
GAGTGCA65650.022.0432936
TGTACCG1650.002709279521.8173125
GTGTAAG10400.021.5206721
GGCTAGA15450.021.4959581
GGAGTGC61150.021.310685
GTATATA7300.020.7693961
GTGTTAC9800.020.6281091
GTATAGA6300.020.6281091
TACACCG2104.524293E-420.5706085
Produced by FastQC (version 0.11.5)