FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671762
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11050.029.3202048
GTCTCGC12400.029.1145671
GTCTTGC24300.027.0394061
GTACTAG4400.026.2560481
CTGTCGC13450.025.6942359
GTGTAAA13650.025.3904631
TCGCTCT12950.025.0212084
GTATAAG7200.024.0680451
GTGTTAC8600.023.5083221
TTGCTCT33450.023.2484044
CTTGCTC30600.023.0605773
GTATTAA6750.022.4635071
TCTCGCT15800.022.3358292
GTGTAAG12050.021.5713581
CTCGCTC13800.021.3929333
CGCTCTG12950.021.1266485
GTATAAC4801.4551915E-1121.059541
TATACTG7450.020.2945655
GCGTTAG2155.2005774E-420.149991
TCTGTCA32700.019.3754448
Produced by FastQC (version 0.11.5)