FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2671762
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11950.037.358291
TCGCTCT12100.033.3221744
TCTGTCG10300.032.8542338
TCTCGCT14350.027.5957032
CGATACT1050.00797569827.4284574
CTCGCTC13750.026.7053453
CGCTCTG13550.026.5681555
GTCTTGC23750.025.1638851
GGGTTAT6200.024.3888231
CTGTCGC15000.023.9998999
GTACTAG4104.1836756E-1122.8308831
GTATAAC4307.8216544E-1121.768981
GTATTAT6650.021.6556661
GTATAGG6100.021.2474021
GTACTAC3758.416464E-921.1214921
GTACTAA5201.8189894E-1220.77071
GTATAAG7750.020.4401551
GTCTCAC26850.020.3813291
GTCTCGA24450.020.3189356
TTACACT11400.020.2104424
Produced by FastQC (version 0.11.5)