FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3909108
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATCG5500.027.99890570
TCTGTCG9250.027.2432218
CTGTCGC12400.021.45169
TAGTACG1154.113851E-521.310334
GTATAAT6000.021.0053581
AGTACGG1408.239562E-620.0056155
AATCGCG1751.267963E-719.99921870
GTATTAT6600.019.095781
GTATTAG7700.019.095781
TGTCGCC12650.019.09040610
ATCTCGT11400.018.42033470
GTCGCCC12000.018.08262611
GTATATA7600.017.9651091
GTCTTAC9600.017.8691431
GACGCTA2259.420546E-817.11044170
GTCTCGC10850.017.1011371
GTATAAG6150.017.0775261
GTATTAA6800.016.9896281
CACGAGC9200.016.73847670
GTCTTGC21350.016.7255781
Produced by FastQC (version 0.11.5)