FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3752060
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9550.027.4864948
GACGATA2900.024.13852970
TGTCGCC11850.021.56085410
CTGTCGC12250.021.1425369
GTCTCGC11200.020.316251
TCGCTCT11450.019.2573494
GTCTTGC22650.019.1646881
CGACGAA4450.018.87687170
GTCGCCC11500.018.8695311
GCGTGTA27150.018.17724670
CGCGTGT37050.018.0436370
GTATAAT5050.018.023131
GTATCCG1200.001340175717.5032331
GTCGTAC1401.864464E-417.5032311
ACGCGTG49150.017.30459870
TAATACT6900.017.2461134
CTCGCTC12550.017.0121463
GTATTGG8300.016.8705861
TCTCGCT13300.016.8440932
GTGTAAG10450.016.7495061
Produced by FastQC (version 0.11.5)