FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4149199
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10350.023.3326558
TCTCGTA7250.023.1717470
ATCTCGT14600.022.05415370
TATACCG1055.4611696E-420.004725
GTCTTGC23750.019.7527451
GCTAATG16100.019.56464870
CTAATCG1801.7060484E-719.4438870
TCGCTCT12250.018.5758134
CTGTCGC14750.018.5079369
GTCTCGC12850.018.2539961
GTATTAG6450.017.9118521
TGTCGCC15100.017.383610
GTATAAC4900.017.1475261
GTATAAG7350.017.1475241
CGCTCTG13350.016.5207545
GTCGCCC14100.016.38250211
GTATTAT5800.016.2975391
ATACCCG2151.0963686E-616.2829134
GTATTAA6050.015.6240871
GTACTAC3855.456968E-1215.4587541
Produced by FastQC (version 0.11.5)