FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4149199
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10550.022.227718
GTATAAG6100.018.364381
GTCTCGC12050.018.0119511
GTATTAG6850.017.8868391
GTCTTGC25500.017.8467561
GTATTAT7000.017.503551
GTATAGA6800.016.9887391
CTGTCGC14500.016.6551389
TGTCGCC14500.016.65513810
CGCCGTA2753.085006E-916.5454268
TGTAGAT23900.016.25500170
TCGCTCT13200.015.9092514
TAGGTGT53600.015.86731270
GGTATAC4200.015.8365451
GTCGCCC13200.015.64409611
TAGACCG1350.002948380315.5555235
GTGTTAG15700.015.3852851
ATAATAC5750.015.2179113
GTAGATC12850.014.98033370
GTTCTAT8000.014.8780171
Produced by FastQC (version 0.11.5)