FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4189047
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG13000.029.345998
CTGTCGC15950.026.332149
TGTCGCC16100.025.4346410
GTCTTGC27850.024.133451
GTCGCCC16000.023.62514911
TCGCCCA16450.022.55306412
CGTTTCG2207.2759576E-1222.2726029
GTCTCGC14000.021.7537731
ATACCCG2201.5643309E-1020.681954
TCGCTCT13800.020.2899844
TACCCGT2555.4569682E-1119.2160365
GTGTAAA15650.019.0128821
TCGAACG1501.4980869E-518.66656113
GTATTAG7250.018.348011
TTGCTCT35500.018.2395524
CGTGTTA5200.018.17297410
CGAACGA1551.9867372E-518.06441314
GGGTTTC38250.018.029271
GTATATA8800.017.5030351
TCTCGCT15850.017.4461542
Produced by FastQC (version 0.11.5)