FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3724418
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9700.031.0303348
AATACCG906.169106E-627.2286455
GTCTCGC10750.025.0756841
TGTCGCC11900.024.70540610
CTGTCGC12850.024.240789
TCGCTCT13050.021.192744
GTCGCCC12300.020.7716811
GTATTAA6500.019.9277781
TCTCGCT12900.019.8108752
GTCTTGC22350.019.7361981
TCGCCCA13700.019.41542612
GTATAGA5600.019.3795721
GTATAGG5400.018.8007321
CGCTCTG13450.018.2199125
GTATAAT6150.018.215681
CTCGCTC13900.017.8816763
GTATATG8250.017.8223861
CGTGTTA4450.017.30303810
TAATACT7000.017.0040114
GTGTTAG16200.016.8558271
Produced by FastQC (version 0.11.5)