FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4189047
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACTAT1500.035.00023370
TCTGTCG10800.022.3607258
GGTATAC4450.020.4542141
CGACGAA1558.7600347E-720.32271670
GTCTCGC12100.019.9633371
TCGCTCT12150.019.300314
GTATTAG8050.019.1349031
ACGGTGT40700.018.74705370
CGGTGTA27800.018.63321770
TGTCGCC13450.018.4755210
GTATACG1551.9826546E-518.0687331
CTCGCTC13150.017.5668683
CTGTCGC13800.017.4996999
GTCGCCC13500.017.3702811
GTATAGG6350.017.0906031
GTCTTGC24150.016.9604821
GTACTAC4750.016.9513261
GTACGGA2552.1746018E-816.4744361
GTATAGA6800.016.4744341
GTGTTAG16200.016.4235861
Produced by FastQC (version 0.11.5)