FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4375072
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCGTA8400.024.58257370
ATCTCGT14250.021.61336970
GTGTAAT9850.018.4803891
TCTGTCG10050.018.4577758
GTCTTGC25250.017.6070381
GTATAGA7000.017.5030611
GTATATA8850.017.0086231
GTATTAT8050.016.9594881
GTGTAAA15400.016.8211231
GTCTCGC11800.016.6130731
TCGCTCT12050.016.5589124
GGCGTAC1704.3838343E-516.4734691
TAATACG1300.002289504516.1566724
GTCTAGC13000.015.8873941
CGTAGAC1554.053702E-415.8088543
GTCTTAC10000.015.7527531
GTCTACG1807.147501E-515.5582771
GTGTAGA12500.015.4026941
CTGTCGC12750.015.37260159
GTACTAA5250.015.3360161
Produced by FastQC (version 0.11.5)