FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4375072
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATTA4100.023.90231770
TCTGTCG11050.022.1720878
GTCTCGC13400.021.6824651
TCGCTCT13650.020.5131864
CTGTCGC12700.019.015879
TCTCGCT15200.018.65252
TGTCGCC13000.018.3078110
GTCGCCC11850.018.01699311
GTCTTGC23200.017.6535971
CTCGCTC15250.017.213423
TCGCCCA13450.017.17483112
CGCTCTG15350.016.8728795
GTATTAA7300.016.7834221
GTATAGA6750.016.5951631
GTAGATC15200.016.1183470
GTATATA9450.015.9283941
GTATAAC4450.015.7327761
GGTATAC4900.015.7167211
GTGTACG2051.2588047E-515.3682341
TGTAGAT25550.015.34238770
Produced by FastQC (version 0.11.5)