FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177602
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11900.026.7638038
TGTCGCC13750.024.18100410
CTGTCGC14250.023.5781549
GTCTCGC12750.023.340371
GTCGCCC13150.022.88896411
TCGCCCA13950.022.07810812
TAATACG800.002664942821.879764
TCGCTCT13500.020.7452564
GTCTTGC25200.019.5892371
GTATAGA7650.018.7638261
GTATAAA10550.018.5838011
CTCGCTC15350.018.4730763
TCTCGCT15050.018.3767532
GTGTTAG16900.018.2303471
GTATTAA7650.017.3908631
GTACTAG5050.017.3319571
GTATAAT7750.016.7147161
GTATTAG8050.016.5267221
TTATACT8900.016.520454
GTATTAC7050.016.3879181
Produced by FastQC (version 0.11.5)