FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177602
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14050.027.6580371
TCTGTCG12800.027.0698328
TCGCTCT12850.026.1483334
TGTCGCC14050.024.41237610
TCTCGCT14400.023.823872
CTGTCGC14850.023.332929
CTCGCTC14100.021.8451523
GTCTTGC27800.021.6599941
GTATTAT7700.021.3688831
GTCGCCC13250.021.131711
GTATTAG6900.019.2800961
GGTATAC5700.019.0397621
GTATTAA7750.018.97241
TCGCCCA15150.018.94355812
CGCCGTA1852.2759559E-718.91858168
ACCGTGT7250.018.8272518
GTGTAAA15450.018.8072071
CGCTCTG16050.018.317655
GTCGTAG1752.7932074E-618.0044211
GTCTTAC11300.017.9690131
Produced by FastQC (version 0.11.5)