FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3556345
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATATCG1601.8189894E-1228.4375470
GTCTCGC9250.027.629431
TCTGTCG9800.024.6418538
GTATAAG5800.024.1447541
TCTCGTA7050.023.33336670
TATCTCG14350.022.92686370
TGTCGCC11400.022.41136610
TCTCGCT11400.022.1115112
TCGCTCT12350.022.11124
CTGTCGC11950.021.379889
GTCGCCC12050.020.3311211
GTATTAA6250.019.6055411
TCGCCCA13200.019.3552712
GTCTTGC21200.018.9912171
CTCGCTC13100.018.9745623
GTATAAC3900.018.8514821
GTATAAT5600.018.75531
GTCGTAC950.007201745618.4262621
TCGTACA1150.001006671118.2660312
CGCTCTG13550.018.0860375
Produced by FastQC (version 0.11.5)