FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508804
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10450.028.468268
CTGTCGC13950.023.0819179
GTCTCGC12900.023.0681481
TGTCGCC14900.021.14046510
TATCTCG16300.020.18381970
GTATTAG9550.019.7958371
TCGCTCT13350.019.6677134
GTCTTGC27800.019.3936441
GTCGCCC14850.018.85458611
TCGCCCA15600.018.6211712
GTATAAG7900.018.6125431
GTATAGG6800.018.5343381
GTATTAT6700.018.2884431
TCTCGCT14550.018.0458352
GCGTAGA2357.925337E-917.8770941
ATCTCGT13850.017.6893370
GTATAAA9500.017.6889131
GTATAGC5850.017.3550421
CTCGCTC15900.017.1738053
GTATACG1704.3804685E-516.4749681
Produced by FastQC (version 0.11.5)