FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4508804
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG14050.025.6582368
CTGTCGC17100.022.7191879
GTCTCGC13150.022.627731
TGTCGCC16700.021.1675610
GTCTTGC28550.020.7218421
GCGTAAC1205.7143603E-520.4204161
GTATAAG7250.020.2795851
GTATAGA6200.019.7616941
TCGCCCA16300.019.75472312
GTCGCCC15550.019.58211311
TCGCTCT15300.017.8434454
TCTCGCT15950.017.5561352
GTATAAT6650.017.371611
TTGCTCT36800.016.739424
GTACGTA1704.383553E-516.4736141
GTCTCAC32550.016.4546341
GTATTAG7750.016.2610511
GCGCTAC1957.832132E-616.1568151
GTGTATA11000.015.9120121
GGGTACG2201.3943736E-615.9120111
Produced by FastQC (version 0.11.5)