FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590573
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9550.025.653688
CGTTTCG1253.095869E-622.3993289
CTGTCGC11800.022.2450949
TGTCGCC12050.021.20268210
GTCGCCC11350.020.96853311
GTATTAG6100.020.6591451
GTCTCGC10800.020.0959051
GTATATA7900.019.9399991
GTCTTGC21850.019.225141
TCGCCCA13400.018.80540512
GTATTAT6600.018.5636671
GTACTAT4950.018.3868711
TCGCTCT11250.018.3583284
GTATAAT5450.017.9846171
GTATAGC5500.017.821121
ATAATAC4750.016.9499283
GTGTTAG14900.016.4456651
GCTATCG1503.1618672E-416.33306970
TAATACT6250.016.2424534
GTGTAAA11700.016.1565111
Produced by FastQC (version 0.11.5)