FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3138708
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9450.022.9622848
GTCTCGC9650.022.4939061
TGTCGCC11250.021.1549310
CTGTCGC11450.021.091089
GTCTTGC18400.020.7400191
ATCTCGT9850.020.60885670
TCGCTCT10550.019.577564
GTCGCCC11050.018.6872311
GTACTAA3600.018.4778581
GTATTAG4450.018.0954071
GTCCTAC6400.018.052381
GTGTTAG12750.017.848581
TCGCCCA11600.017.80119712
GTATTCG1602.6029145E-517.5053391
CGTGTTA2802.0736479E-1017.49948110
GTTAGCC10250.017.4182823
TAACACT9000.017.1146954
CGCTCTG11100.017.0305925
GTATTAC3950.016.8405781
CGTTTCG1250.001763672516.7997727
Produced by FastQC (version 0.11.5)