FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3936142
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8850.026.8917248
CTGTCGC12550.020.915619
TGTCGCC12800.019.13996310
GTCGCCC11400.019.03442811
GTATAGG6350.018.7452221
GCGTAGA2254.623871E-918.6717111
GTATTAG7650.018.30561
ATCTCGT11050.018.05390470
GTCTTGC21450.017.3007131
TATACCG1250.001760583516.8043275
TCTCGTA6700.016.71605170
GGTATAC4500.016.3377481
TCGCTCT11100.016.0852244
TCGCCCA13850.015.92002512
GTCTCGC10150.015.8663581
GTGTTAG15200.015.4318031
GTATTAT5900.015.4278981
TAAGATG14900.015.26812370
GTATAAG6050.015.0453871
ACTGTCG2806.888513E-814.999488
Produced by FastQC (version 0.11.5)