FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344184

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344184
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3936142
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9650.024.300358
GTCTCGC10900.024.086381
TCGCTCT12700.020.669154
CTGTCGC12150.020.4525349
TCTCGCT12500.020.4416752
GTCTTGC23250.019.2718661
TACCCGT2002.4469045E-819.2498665
TGTCGCC11700.019.14516610
GTCGCCC10700.018.3176311
GTATAGG6450.017.3670881
CGCTCTG15450.016.7636385
GTATTAT7100.016.7632161
GTATATA7800.016.6051921
CTCGCTC14600.016.5416133
GTATTAG6350.016.538051
TCGCCCA12500.016.23988712
GTATAGA7850.016.0534951
GGTATAC3950.015.9518921
GTCTCAC27000.015.29871751
GTTCTAC7350.015.2405061
Produced by FastQC (version 0.11.5)