FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3719309
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10250.029.7069688
GTCTCGC11400.027.3279171
CTGTCGC12200.026.106259
TGTCGCC12250.024.5711410
GTCTTGC24550.023.954061
GTCGCCC11500.023.43482411
TCGCTCT11800.023.1359444
CGATACA3800.023.02635670
TCGCCCA12150.022.18110812
TCTCGCT13450.021.3394762
CTCGCTC13300.020.790073
GTATTAC5300.020.474211
GTATTAT7100.020.2137491
GGGTTTC35650.019.5395661
ACGATAA2900.019.31037770
GTACGCT1107.4806577E-419.0911984
GTACTAT5050.018.7151681
CGACGAA4900.018.5714670
GTGTACG2659.458745E-1118.4928361
GTGTTAG15750.018.4467091
Produced by FastQC (version 0.11.5)