FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3604570
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG2400.033.54149270
GCGAATG5400.027.22208270
TCTGTCG10950.026.2091858
GTATTAT5800.025.9560641
CTGTCGC13600.023.4183489
GTCTCGC10950.023.3403361
TCGCTCT11500.023.1357734
TGTCGCC12750.022.50906410
GTCGCCC12950.020.26960211
CTCGCTC13100.019.7755553
CGCTCTG12450.019.6832545
TCTCGCT12900.019.5402052
GTCTTGC22000.019.4149171
GTATTGA6900.019.2811471
GTGTAAC7600.018.426581
TCGCCCA14650.018.395312
GTATTAA7450.017.3877681
GTATTAG6650.017.3736341
GTATAAT6050.017.360581
GTGTTAT8650.016.999321
Produced by FastQC (version 0.11.5)