FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4483672
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12400.027.661088
CTGTCGC14950.024.5817539
TGTCGCC15500.022.58047110
GTCGCCC14500.022.20623211
TCGCTCT13750.021.3876154
GTCTTGC26850.021.123281
GTCTCGC14250.020.883091
ATCTCGT12250.019.14249670
GTATTAG9000.019.0609381
TCGCCCA16450.018.72284512
CTCGCTC15200.018.6559583
CGCTCTG15950.017.998615
TCTCGCT15950.017.5592292
TAATACT9400.017.5047474
GTATTAC6050.017.3602711
GTACTAC5250.017.3382281
GTATAGA7150.017.1377051
GTATAGG5950.017.063641
TCTCGTA7800.015.70483370
TTGCTCT35400.015.6257074
Produced by FastQC (version 0.11.5)