FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4281682
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11550.028.7870968
TCGCTCT12900.024.9672684
GTCTCGC14100.023.8360981
TGTCGCC14950.023.17662610
CTGTCGC15350.023.0286889
GTATTAG7750.022.1348921
GTCTTGC26800.021.5542141
GTCGCCC14650.021.50112211
TCTCGCT15650.021.4748332
CTCGCTC15500.021.005073
CGCTCTG14850.020.745755
TCGCCCA16850.020.5632412
GTGTTAC9400.019.7392671
GTATAGT7050.018.8702431
GTATTAT7150.018.6063231
GGTATAC4750.018.425931
TAATACT8400.017.9209944
GTATAGA7500.017.738031
CGAACGA1200.001342122217.49952314
GTACTAG5500.017.1863691
Produced by FastQC (version 0.11.5)