FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4281682
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12150.027.366718
GTCTCGC13400.025.3412741
TGTCGCC14800.023.88525410
TCGCTCT14100.023.333454
CTGTCGC15350.023.0294329
GTCGCCC13600.021.36065111
TCTCGCT15000.020.768712
CGCTCTG14750.019.9323045
GTCTTGC27900.019.0722061
TCGCCCA14900.018.08755112
CTCGCTC16550.017.976133
GTCTTAC10350.017.9265631
GTATAAG8800.017.901581
GTGTAGG12550.017.8524461
GTATAGA6900.017.7574441
GCGTAAT1401.8641054E-417.5037671
TATACTG8950.016.4246645
GTGTTAG17600.016.3103281
GTGTTAC11050.016.1573241
TTGCTCT33600.016.1459144
Produced by FastQC (version 0.11.5)