FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4294151
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG15550.040.2888578
GTCTCGC16750.032.6761051
CTGTCGC20200.032.440399
GTCTTGC34650.032.4229741
TCGCTCT17750.031.233654
CTCGCTC19550.028.3632013
GTGTTAC17350.027.8103731
TCTCGCT19650.027.1194232
GGGTTTC59300.024.1673971
CGCTCTG21050.023.9470585
GTCTTAC13700.023.6550271
GTGTAAA25100.023.5272751
GTCTAAC8350.023.2867451
GTTTCAC53800.022.888913
CTTGCTC45150.022.3296323
TTGCTCT45900.022.27444
GTCTAGA15550.020.8407631
GTATTAG14350.020.576121
GTCTAGC16600.020.3901921
GTACCGG3901.3200406E-820.3123231
Produced by FastQC (version 0.11.5)