FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4294151
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC16050.035.8986431
TCGCTCT18050.033.5052954
TCTGTCG17900.030.970568
CTCGCTC19800.029.4531143
GTCTTGC32600.029.1621441
CGCTCTG19900.027.8579435
TCTCGCT21750.025.8215392
GTCTTAC15100.024.8021581
GGGTTTC58850.023.252531
GTCTCAC40150.022.9608271
GTATTAA12300.022.8361321
GTGTTAC15450.021.9094981
TTGCTCT46350.021.7465154
GTGTAAC11400.021.4801431
GTCTAGC16100.021.4722941
GTGTAAG19200.021.3814281
GTTTCAC53800.021.1439533
GTGTAAA25250.020.8221021
GTATTAG12250.020.5776141
TCTGTCA46000.020.503358
Produced by FastQC (version 0.11.5)