FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3168879
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12050.046.605318
GTCTCGC14100.042.429391
TCGCTCT13900.040.4024434
CTGTCGC15650.038.6449559
CGCTCTG16350.034.3482555
TCTCGCT16600.034.264692
GTCTTGC24200.033.3588031
CTCGCTC16950.032.2828373
GTCTTAC9900.030.5789011
GTGTTAC10900.029.7573181
TTGCTCT33000.025.9633584
GGTATAC6150.025.7842981
GTATTAG9950.025.3543641
GGGTAAT10050.024.384881
GTCTCGA27350.024.2191186
TAATACT7850.023.8468784
GGGTTTC44700.023.5425281
GTATAGA10200.023.3196241
GAGTGCA74300.023.2568176
CTGTTGC28600.022.4053549
Produced by FastQC (version 0.11.5)