FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3063028
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC12950.043.9240261
GTCTTGC26850.037.8111271
TCGCTCT14650.035.386094
CGCTCTG15850.030.4356945
CTCGCTC16500.029.2367153
TTGCTCT31900.028.4392134
GTGTAAT11300.028.0361941
TCTCGCT19000.027.2845382
GTACTAG5550.027.2440151
GTATTAG10050.027.2246511
GTATTAC6800.026.4713381
CTTGCTC33950.026.2978133
TCTGTCG19100.026.011218
GTCTCGA26050.025.7047256
GTGTAAC8700.025.6559011
ATGGCGT13550.025.505846
GGGTTTC43900.024.7660791
GTGTTAC11650.024.721731
AATGGCG16050.024.6731955
GAGTGCA68100.023.4716456
Produced by FastQC (version 0.11.5)