FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3984358
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13700.032.0594751
GTCTTGC33350.029.3623091
TCGCTCT15550.028.7090844
GTGTTAG21850.026.3624461
TCTGTCG20800.025.6178578
GTCTTAC13550.024.9750121
CGCTCTG16950.023.789015
GTATTAC8800.023.7280731
GTGTTAC14150.023.407151
GTGTAAC11400.023.3692111
GTCTCAC37800.023.2388781
GTTAGCC18300.022.8210143
TTGCTCT42150.022.549374
CTCGCTC18650.022.3927383
GGAGTGC72150.022.3547465
GTACTAA6200.022.065261
GAGTGCA76200.022.0170426
TTACACT18400.021.9143334
GGCTGGA105450.021.3719881
TCGATAC2704.722986E-621.3345873
Produced by FastQC (version 0.11.5)