FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344207

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344207
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510908
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12250.042.905798
GTCTCGC14000.042.2185971
TCGCTCT14300.039.2724234
GTCTTGC29750.035.1316871
CTGTCGC16250.035.0028049
TCTCGCT16250.033.230512
CTCGCTC17250.031.721493
CGCTCTG17150.030.6469925
GGTATAC5800.028.5836621
TAATACT10800.027.3331184
GTCTTAC12150.026.6964931
GTCTAGC15150.025.2162931
CGTTTCG2601.2259261E-724.9228829
GGGTTTC50600.024.5016841
TTGCTCT39450.024.4560834
GTCTCAC36650.024.3874111
GTCTCGA28100.024.3414466
GTATTAG11650.023.5112461
GGAGTGC75650.023.5080785
TTACGCT2151.9702948E-523.4416794
Produced by FastQC (version 0.11.5)