FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005344208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005344208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3510908
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC14450.042.853311
TCGCTCT15300.038.1178864
TCTGTCG19500.033.9708948
GTCTTGC28800.033.751651
CTCGCTC17150.033.1663863
CGCTCTG17000.032.611975
TCTCGCT19100.029.4029062
GTCTCAC34050.028.9705811
TTGCTCT41600.026.3078564
GGGTTTC49300.025.1209071
GTCTTAC11600.024.2080821
GTTTCAC45100.023.7872873
CTTGCTC40200.023.641943
GTACTAC6450.023.4430071
GTGTTAG22500.023.3611451
CTGTTGC34950.023.0740919
GTTAGCC17300.022.8903163
GTCCTAC9450.022.8582611
TCACTCT44000.022.4183224
TCTGTCA37100.022.3191538
Produced by FastQC (version 0.11.5)